Regenerative medicine involves the use of cells, biological tissues and organs for medical and biotechnological applications. Bioprinting Applied to Regenerative Medicine covers topics including 3D printing technology, biomaterials, cell culture, biomanufacturing and tissue engineering. However, the operation of the bioprinting machine needs to be kept in a sterile state in a clean room, but the clean room is expensive. Micro-environment-type clean spaces are suitable for providing ultra-clean environments for single devices, such as bioprinters. This research is mainly to simulate and compare the impact of environmental pollution of self-installed bioprinters in different cleanliness environments, and analyze it by the colony method. The research results show that when the printing machine is in a good microenvironment, it can achieve 100% colony-free formation. Therefore, the integration of the environment-type clean space and the bioprinting machine can not only minimize the operating space, but also avoid the formation of microorganisms during the bioprinting process.
